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phospho akt2 ser474  (MedChemExpress)


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    Structured Review

    MedChemExpress phospho akt2 ser474
    Phospho Akt2 Ser474, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+akt2+ser474/AKT1%2BAKT2%2BAKT3+Antibody/pm41437043-159-0-30
    Average 94 stars, based on 2 article reviews
    phospho akt2 ser474 - by Bioz Stars, 2026-09
    94/100 stars

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    other:

    Article Title: Trained immunity attenuated acute lung injury by activating alveolar macrophages via AKT2-PDK1 axis-mediated metabolic reprogramming.
    Article Snippet: Phospho-AKT2 (Ser474) (AF3264), PDK1 (DF4365), TNF-α (AF7014), IL-6 (DF6087) antibodies are purchased from Affinity Corporation (Changzhou, China). β-actin (81115-1-RR), CD86 (13395-1-AP), CD206(32647-1-AP) from Proteintech (Wuhan, China), CCT128930 and AZD7545 from MCE (New Jersey, USA).

    Article Title: Trained immunity attenuated acute lung injury by activating alveolar macrophages via AKT2-PDK1 axis-mediated metabolic reprogramming
    Article Snippet: Phospho-AKT2 (Ser474) (AF3264), PDK1 (DF4365), TNF-α (AF7014), IL-6 (DF6087) antibodies are purchased from Affinity Corporation (Changzhou, China). β-actin (81115-1-RR), CD86 (13395-1-AP), CD206(32647-1-AP) from Proteintech (Wuhan, China), CCT128930 and AZD7545 from MCE (New Jersey, USA).



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    Analysis of hepatic SerpinA3k expression, lipid accumulation, and related parameters. ( A ) Representative blots and densitometric analysis of SerpinA3k abundance in the liver of wild-type (WT) animals fed a standard chow diet (SD), high-fat diet (HFD) or with type 2 diabetes (D2), normalized by β-actin. ( B to G ) Representative hepatic histomorphology images showing lipid droplets (200X magnification) in WT and SerpinA3k knockout (KO) mice on SD, HDF or type 2 diabetes as stated. ( H ) Relative frequency (percentage) of lipid droplets area stratified by sizes. ( I ) Relative frequency (percentage) of lipid droplets ranging from 8000 to 12000 μm 2 . ( J ) Relative frequency (percentage) of lipid droplets ranging from 1 2 000 to 40000 μm 2 . ( K ) Triglycerides content in the liver. ( L ) Representative blots and densitometric analysis of the <t>pAkt2</t> Se474/Akt ratio in the liver of WT and KO mice, normalized to β-actin. Relative mRNA abundance of genes coding for: ( M ) Srebp-1c , ( N ) Pck1, ( O ) Srebp2 , ( P ) Hmgcs1 , and ( Q ) Hmgcr CoA normalized by m36B4 and b-2 microglubulin. Data are presented as mean ± SD. * p <0.05, ** p <0.01, *** p <0.001, and **** p <0.0001 vs. their respective WT+SD or KO+SD controls as stated; ++++ p <0.0001 WT+D2 vs. KO+D2 mice; # p <0.05 and ## p <0.01 KO+HFD vs . KO+D2; and % p <0.05, WT+HFD vs. KO+HFD, as stated
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    Analysis of hepatic SerpinA3k expression, lipid accumulation, and related parameters. ( A ) Representative blots and densitometric analysis of SerpinA3k abundance in the liver of wild-type (WT) animals fed a standard chow diet (SD), high-fat diet (HFD) or with type 2 diabetes (D2), normalized by β-actin. ( B to G ) Representative hepatic histomorphology images showing lipid droplets (200X magnification) in WT and SerpinA3k knockout (KO) mice on SD, HDF or type 2 diabetes as stated. ( H ) Relative frequency (percentage) of lipid droplets area stratified by sizes. ( I ) Relative frequency (percentage) of lipid droplets ranging from 8000 to 12000 μm 2 . ( J ) Relative frequency (percentage) of lipid droplets ranging from 1 2 000 to 40000 μm 2 . ( K ) Triglycerides content in the liver. ( L ) Representative blots and densitometric analysis of the <t>pAkt2</t> Se474/Akt ratio in the liver of WT and KO mice, normalized to β-actin. Relative mRNA abundance of genes coding for: ( M ) Srebp-1c , ( N ) Pck1, ( O ) Srebp2 , ( P ) Hmgcs1 , and ( Q ) Hmgcr CoA normalized by m36B4 and b-2 microglubulin. Data are presented as mean ± SD. * p <0.05, ** p <0.01, *** p <0.001, and **** p <0.0001 vs. their respective WT+SD or KO+SD controls as stated; ++++ p <0.0001 WT+D2 vs. KO+D2 mice; # p <0.05 and ## p <0.01 KO+HFD vs . KO+D2; and % p <0.05, WT+HFD vs. KO+HFD, as stated
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    Image Search Results


    Analysis of hepatic SerpinA3k expression, lipid accumulation, and related parameters. ( A ) Representative blots and densitometric analysis of SerpinA3k abundance in the liver of wild-type (WT) animals fed a standard chow diet (SD), high-fat diet (HFD) or with type 2 diabetes (D2), normalized by β-actin. ( B to G ) Representative hepatic histomorphology images showing lipid droplets (200X magnification) in WT and SerpinA3k knockout (KO) mice on SD, HDF or type 2 diabetes as stated. ( H ) Relative frequency (percentage) of lipid droplets area stratified by sizes. ( I ) Relative frequency (percentage) of lipid droplets ranging from 8000 to 12000 μm 2 . ( J ) Relative frequency (percentage) of lipid droplets ranging from 1 2 000 to 40000 μm 2 . ( K ) Triglycerides content in the liver. ( L ) Representative blots and densitometric analysis of the pAkt2 Se474/Akt ratio in the liver of WT and KO mice, normalized to β-actin. Relative mRNA abundance of genes coding for: ( M ) Srebp-1c , ( N ) Pck1, ( O ) Srebp2 , ( P ) Hmgcs1 , and ( Q ) Hmgcr CoA normalized by m36B4 and b-2 microglubulin. Data are presented as mean ± SD. * p <0.05, ** p <0.01, *** p <0.001, and **** p <0.0001 vs. their respective WT+SD or KO+SD controls as stated; ++++ p <0.0001 WT+D2 vs. KO+D2 mice; # p <0.05 and ## p <0.01 KO+HFD vs . KO+D2; and % p <0.05, WT+HFD vs. KO+HFD, as stated

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: SerpinA3k Deficiency Ameliorates Experimental type 2 Diabetes

    doi: 10.1007/s00018-025-05922-3

    Figure Lengend Snippet: Analysis of hepatic SerpinA3k expression, lipid accumulation, and related parameters. ( A ) Representative blots and densitometric analysis of SerpinA3k abundance in the liver of wild-type (WT) animals fed a standard chow diet (SD), high-fat diet (HFD) or with type 2 diabetes (D2), normalized by β-actin. ( B to G ) Representative hepatic histomorphology images showing lipid droplets (200X magnification) in WT and SerpinA3k knockout (KO) mice on SD, HDF or type 2 diabetes as stated. ( H ) Relative frequency (percentage) of lipid droplets area stratified by sizes. ( I ) Relative frequency (percentage) of lipid droplets ranging from 8000 to 12000 μm 2 . ( J ) Relative frequency (percentage) of lipid droplets ranging from 1 2 000 to 40000 μm 2 . ( K ) Triglycerides content in the liver. ( L ) Representative blots and densitometric analysis of the pAkt2 Se474/Akt ratio in the liver of WT and KO mice, normalized to β-actin. Relative mRNA abundance of genes coding for: ( M ) Srebp-1c , ( N ) Pck1, ( O ) Srebp2 , ( P ) Hmgcs1 , and ( Q ) Hmgcr CoA normalized by m36B4 and b-2 microglubulin. Data are presented as mean ± SD. * p <0.05, ** p <0.01, *** p <0.001, and **** p <0.0001 vs. their respective WT+SD or KO+SD controls as stated; ++++ p <0.0001 WT+D2 vs. KO+D2 mice; # p <0.05 and ## p <0.01 KO+HFD vs . KO+D2; and % p <0.05, WT+HFD vs. KO+HFD, as stated

    Article Snippet: USA), pAKT2 Ser474 (1,2,000; Cat. No, 8599 S, Cell Signaling, MA, USA), totalAKT2 (1:2,000; Cat. No. 3063 S, Cell Signaling, MA, USA) and interleukin 6 (1:1000, Cat. No. SABS700632, Sigma, USA).

    Techniques: Expressing, Knock-Out